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保育研究

研究生補(捐)助

計畫期限 2016-01-01 ~ 2017-08-31
研究名稱 研究東沙群島中海草種子之形態及主要蛋白質組成
受託單位 國立中興大學
計畫主持人 Buntora Pasaribu
協同主持人
計畫顧問 曾志正 教授
參與計畫人員
關鍵詞 Seagrass seeds、triacyglycerol (TAG)
摘要 Seagrass is a unique flowering plant that plays a key role in the stabilization of coastal ecosystem. Seeds produced by this plant store triacylglycerol as the energy source for germination and post-germinative growth of seedlings. Seeds of mature seagrass Thalassia hemprichii when stained with a fluorescent probe BODIPY showed the presence of oil bodies in intracellular cells and after further lipid analyses, triacyglycerol (TAG) was found to be a major lipid classes in seagrass seeds. Protein extracted from seagrass seeds were subjected to immunological cross-recognition with land plant seed oil body proteins (oleosin; caleosin) and found to have no cross-reactivity. Oleosin-like gene was found in seagrass seeds. Next generation sequencing and sequence alignment indicated that the deduced seagrass seed oleosin-like protein has a central hydrophobic domain responsible for their anchoring onto the surface of oil bodies. Phylogenetic analysis showed that the oleosin-like protein was evolutionarily closer to pollen oleosin than to seed oleosins. Thus, oil body protein found in seagrass oil bodies seemed to represent a distinct class from land seed oil body proteins.
檔案1 研究東沙群島中海草種子之形態及主要蛋白質組成  PDF 下載次數 91
檔案2 研究東沙群島中海草種子之形態及主要蛋白質組成  PDF 下載次數 92
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